倒立超解析白光雷射共軛焦顯微鏡 (TCS SP8X STED)

。研究發表 。設備聯絡人 　姓名：陳志俊 　　　　梅秀富 　　　　黃美綾 　電話：(03)3281200 　分機：7706

技術原理 儀器規格 機型：Leica TCS SP8X STED 光源： Visible laser White light laser system: pulsed laser super continuous exciting range: 470nm-670nm 1nm adjustable >1mW for each wavelength UV laser 405nm laser 50mW 顯微鏡：LEICA DMi8 倒立顯微鏡 壹台 物鏡： Plan Fluotar 5x, dry, NA 0.15, WD≧13.7mm Plan APO 10x, dry, NA 0.40, WD≧2.2mm Plan APO 20x, NA 0.70, coverglass, WD≧0.59mm Plan APO 40x, oil, NA 1.30, WD≧0.24mm Plan APO 63x, oil, NA 1.40, WD≧0.14mm Plan APO 63x, glyc, NA 1.30, CORR, WD≧0.3mm Plan APO 93x, glyc, NA 1.30, motC STED WLL , WD≧0.3mm Plan APO 100x, oil, NA 1.40, STED WHITE , WD≧0.09mm 掃瞄器： for UV/405 nm-VIS-IR-gSTED with 4 independent laser ports Light gate FLIM technology High resolution scan mode: Scan field of view(FOV) at least 22 mm, speed 7fps@512x512 in FOV 22mm, 84fps @ 512 x 16 Max. scan resolution 8192 x 8192, 16 bits grey scale, digitalization 12bits or 18 bits. CCD： 1.4 Mpixel (1392 x 1040) Exposure time 4us-10min 影像擷取形式：  ◆ Bright field  ◆ Fluorescence  ◆ Reflection  ◆ Normaski differential interference contrast (DIC) Build-in Scanner' confocal detector：  Multi-band Spectrophotometer（400-850 nm）with 4PMT tube (2 cooling hybrid GaAsP/APD detectors + 2 cooled PMT detectors) 3D STED module 592nm STED laser 660nm STED laser 775nm STED laser Resolution dxy~30nm, dz<100nm 3D STED device Incubation system 軟體： Leica Application Suite X Imaris 3D/4D real time image software Metamorph image analysis software