Page 56 - @林口醫研部201909電子報

撰稿者 / 校稿者 : 林⼝⾧庚醫院神經內科 張國軒醫師

‣應⽤性

細胞重編程製造誘導式多能幹細胞的技術, 是再⽣醫學領域的重⼤突破, 本研究建置的

誘導式多能幹細胞模式克服了取得病⼈腦部存活神經細胞的技術問題, 與胚胎幹細胞的倫理

爭議, 可以應⽤在是神經退化性疾病的疾病機轉與藥物研究上。

‣圖表1

Characterization and neuronal differentiation of AD-iPSCs.

(a) Teratoma formed after subcutaneously injecting AD-iPSCs into nude mice.
(b) Ectodermal (neural tissues and hair follicles), mesodermal (adipose

tissues and cartilages), and endodermal (respiratory and
gastrointestinal epithelium) tissues in the teratoma. Scale bar, 100 μm.

(c) AD-iPSCs were differentiated into neural rosettes. Scale bar, 100 μm.
(d) AD-iPSC-derived neural stem cells expressing NES (red).

(e) AD-iPSC-derived neurons expressing TUBB3 (green). Nuclei were
stained with DAPI. Scale bar, 100 μm.

(f) Relative cell viability and caspase 3 activity of NC-(NC1 and NC2) and AD-
iPSC-derived neurons (AD1 and AD2).

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