Page 44 - @林口醫研部201909電子報

撰稿者:腫瘤醫學研究中⼼ ⾼詩涵博⼠ 校稿者:癌症基因體研究中⼼ 吳國瑞教授

‣圖表4

Nonubiquitinated DDX17 promotes the miRNA biogenesis of a
subset of anti-stemness miRNAs and reduces binding to the
proximal regions of the promoters of the stemnss-related genes.

(A) The miRNA–gene network showing miRNAs targeting to the

stemness-related genes based on experimentally validated evidence
(solid line) and predicted algorithms (dashed line).

(B) qRT-PCR analysis of mature miRNA levels normalized to RNU-48
of DDX17-WT or DDX17-K190R-expressing H1299 cells.

(C) qChIP analysis of BMI1, SOX2, and OCT4 promoters of H1299
cells under normoxia (■) or hypoxia (Δ) by immunoprecipitating

DDX17, p300, H3K56Ac, or YAP with their specific antibodies.
(D) qChIP analysis of BMI1, SOX2, and OCT4 promoters of Flag-

tagged DDX17-WT or DDX17-K190R–overexpressing H1299 cells.

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