Page 41 - 林口醫研部2022三月電子報

•FIGURE 4. Mineralization assay. The hPDLCs cells in (A) control medium,or with

(B) 10n Mcalcitriol, (C) 100 μg/mL EMD, or (D) 100 μM vitamin C. Calcified
regions obtained from SEM imaging and identified by computational program

were pseudo-colored in yellow. (E) The comparison of the percentage of

mineralized areas in the SEM samples (n = 9). The boxplot showed the
percentage of mineralized areas for each condition. The student t-test showed

mineralized areas in all three treatments were significantly greater than that of

the control group (P values < 0.05). (F through I) The sagittal views of the
mineralization in each condition. (J) Comparisons of average mineralization

thickness for each condition (n = 7∼13). (K) Energy-dispersive X-ray element

analysis. Calcium, phosphorus, and silver elements in the mineralized area of
hPDLCs cells were mapped after Von Kossa stain. The results showed that the

mineralization was replaced by silver efficiently during the Von Kossa staining

and the mineralization in three treatments were significantly higher than

control medium (Student t-test showed both phosphorus and silver elements
are significantly different compared with control in all three treatments; P <

0.05)

Department of Medical Research & Development , Linkou Newsletter 41