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臨床蛋白質體核心實驗室
Clinical Proteomics Core Laboratory
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研 究 成 果 |
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重要的研究成果
- 建立快速生物檢體蛋白質譜分析系統 適用於尋找疾病診斷及預後的生物標誌 (SELDI- TOF MS & Magnetic-bead MALDI-TOF MS)
- 建立疾病蛋白質體學及系統生物學研究平台
- 三十篇論文發表國際一流期刊 其中Molecular &Cellular Proteomics (IF;9.6)一篇;J. Proteome Res & Proteomics五篇
- 系統地分析檢體收集及儲存操作流程對血清血漿蛋白質體的影響→建立檢體收集及儲存標準流程
- 證實質譜可運用於高效率的微生物分類及鑑定
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Published in Mol Cell Proteomic 2007; Nov 27, [Epub ahead of print] |
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研究成果
| 論文名稱 |
| 2021 |
| new*Ming-Tse Kuo, Po-Chiung Fang, Shu-Fang Kuo, Alexander Chen, and Yu-Ting Huang. Tear Proteomics Study of Dry Eye Disease: Which Eye Do You Adopt as the Representative Eye for the Study? Int J Mol Sci. 2021 Jan; 22(1): 422. (致謝_高長) |
| new* Wei-Ling Chen, You-Lin Tain, Hung-En Chen, and Chien-Ning Hsu. Cardiovascular Disease Risk in Children With Chronic Kidney Disease: Impact of Apolipoprotein C-II and Apolipoprotein C-III.Front Pediatr. 2021; 9: 706323. (致謝_高長) |
| new* Lien-Hung Huang, Cheng-Shyuan Rau, Shao-Chun Wu, Yi-Chan Wu, Chia-Jung Wu, Chia-Wen Tsai, Chia-Wei Lin, Tsu-Hsiang Lu, Ching-Hua Hsieh, Identification and characterization of hADSC-derived exosome proteins from different isolation methods. J. Cell Mol. Med. 2021 June 21, 25: 7436-7450 (致謝_高長) |
| new* Johnson Chia-Shen Yang,Lien-Hung Huang,Shao-Chun Wu,Pao-Jen Kuo,Yi-Chan Wu,Chia-Jung Wu,Chia-Wei Lin,Pei-Yu Tsai and Ching-Hua Hsieh, Lymphaticovenous Anastomosis Supermicrosurgery Decreases Oxidative Stress and Increases Antioxidant Capacity in the Serum of Lymphedema Patients. J. Clin. Med. 2021, 6 April, 10(7), 1540 (致謝_高長) |
| new* Ken-Pen Weng, Sung-Chou Li, Kuang-Jen Chien, Kuo-Wang Tsai, Ho-Chang Kuo, Kai-Sheng Hsieh and Shih-Hui Huang. Prediction Model for Diagnosis of Kawasaki Disease Using iTRAQ-Based Analysis. Children 2021, 5 July, 8(7), 576 (高長) |
| new* Shui-Yi Tung, Ko-Chao Lee, Kam-Fai Lee, Ya-Ling Yang, Wen-Shih Huang, Li-Ya Lee, Wan-Ping Chen, Chin-Chu Chen, Chih-Chuan Teng, Chien-Heng Shen, Meng-Chiao Hsieh, Cheng-Yi Huang, Jiunn-Ming Sheen, Hsing-Chun Kuo. Apoptotic mechanisms of gastric cancer cells induced by isolated erinacine S through epigenetic histone H3 methylation of FasL and TRAIL. Food Funct. 2021 Apr 21;12(8):3455-3468. (致謝) |
| new* Cheng-Shyuan Rau, Pao-Jen Kuo, Shao-Chun Wu, Lien-Hung Huang, Tsu-Hsiang Lu, Yi-Chan Wu, Chia-Jung Wu, Chia-Wei Lin, Chia-Wen Tsai and Ching-Hua Hsieh. Enhanced Nerve Regeneration by Exosomes Secreted by Adipose-Derived Stem Cells with or without FK506 Stimulation. Int. J. Mol. Sci. 9 Aug. 2021, 22(16), 8545 (致謝_高長) |
| new* Sheng‑Yu Lee, Tzu‑Yun Wang, Ru‑B and Lu, Liang‑Jen Wang, Sung‑Chou Li, Chi‑YingTu, Cheng‑Ho Chang, Yung‑Chih Chiang & Kuo‑WangTsai. Identification of potential plasma protein biomarkers for bipolar II disorder: a preliminary/exploratory study. Scientific Reports, 9452 (2021) 11: 9452 |
| new* Jei-Ming Peng, Ruo-Han Tseng, Tsung-Chieh Shih, Sen-Yung Hsieh. CAMK2N1 suppresses hepatoma growth through inhibiting E2F1-mediated cell-cycle signaling. Cancer Lett. 2021 Jan 28;497:66-76. (致謝) |
| new* Shao-Chun Wu, Pao-Jen Kuo, Cheng-Shyuan Rau, Lien-Hung Huang, Chia-Wei Lin, Yi-Chan Wu, Chia-Jung Wu, Chia-Wen Tsai, Ting-Min Hsieh, Hang-Tsung Liu, Chun-Ying Huang and Ching-Hua Hsieh. Increased Angiogenesis by Exosomes Secreted by Adipose-Derived Stem Cells upon Lipopolysaccharide Stimulation. Int. J. Mol. Sci. 2021 Aug 18, 22(16), 8877 (技術平台) |
| new* Kung-Chuan Cheng ,Hsing-Chun Kuo,Meng-Chiao Hsieh ,Cheng-Yi Huang ,Chih-Chuan Teng ,Shui-Yi Tung ,Chien-Heng Shen ,Kam-Fai Lee ,Ya-Ling Yang and Ko-Chao Lee. Identification of Two Novel CIL-102 Upregulations of ERP29 and FUMH to Inhibit the Migration and Invasiveness of Colorectal Cancer Cells by Using the Proteomic Approach. Biomolecules 2021, 11(9), 1280. (致謝_核心實驗室) |
| new* Chia-Lung Tsai , Shih-Ming Jung , Lang-Ming Chi , Chi-Neu Tsai , Chiao-Yun Lin , Angel Chao , Yun-Shien Lee. Glycogen synthase kinase-3 beta (GSK3β)-mediated phosphorylation of ETS1 promotes progression of ovarian carcinoma. Aging (Albany NY). 2021 May 31.13739–13763.(核心成員) |
| 2020 |
| new* Jei-Ming Peng , Sheng-Hsuan Lin , Ming-Chin Yu , Sen-Yung Hsieh. CLIC1 recruits PIP5K1A/C to induce cell-matrix adhesions for tumor metastasis. J Clin Invest. 2021 Jan 4;131(1):e133525. (致謝_核心實驗室) |
| Sung-Chou Li, Kuo-Wang Tsai, Lien-Hung Huang, Ken-Pen Weng, Kuang-Jen Chien, Yuyu Lin, Chi-Ying Tu & Pei-Hsien Lin, Serum proteins may facilitate the identification of Kawasaki disease and promote in vitro neutrophil infiltration , Scientific Reports (2020) v.10, Article number: 15645 (高長-李松洲_高榮-翁根本,方法) |
| Hsiao YC, Lin SY, Chien KY, Chen SF, Wu CC, Chang YT, Chi LM, Chu LJ, Chiang WF, Chien CY, Chang KP, Chang YS, Yu JS. An immuno-MALDI mass spectrometry assay for the oral cancer biomarker, matrix metalloproteinase-1, in dried saliva spot samples. Anal Chim Acta. 2020 Mar 1;1100:118-130. (核心成員) |
2019 |
| Tang WF, Huang RT, Chien KY, Tang P, Horng JT. Large-Scale Proteomic Identification of Targets of Cellular miR-197 Downregulated by Enterovirus A71.J Proteome Res. 2019 Jan 4;18(1):449-460.
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| 2018 |
| Chien-Ning Hsu, Yu-Ju Lin, You-Lin Tain, Postnatal high-fat diet sex-specifically exacerbates prenatal dexamethasone-induced hypertension: Mass spectrometry-based quantitative proteomic approach, The Journal of Nutritional Biochemistry, V.57, July 2018, Pages 268-275 (高長-田祐霖, 致謝)
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| Hsu CL, Lui KW, Chi LM, Kuo YC, Chao YK, Yeh CN, Lee LY, Huang Y, Lin TL, Huang MY, Lai YR, Yeh YM, Fan HC, Lin AC, Lu YJ, Hsieh CH, Chang KP, Tsang NM, Wang HM, Chang AY, Chang YS, Li HP. Integrated genomic analyses in PDX model reveal a cyclin-dependent kinase inhibitor Palbociclib as a novel candidate drug for nasopharyngeal carcinoma. J Exp Clin Cancer Res. 2018 Sep 20;37(1):233. . |
| ang TY, Chi LM, Chien KY. Size-exclusion chromatography using reverse-phase columns for protein separation. J Chromatogr A. 2018 Oct 12;1571:201-212. |
| Hsiao YC, Chu LJ, Chen YT, Chi LM, Chien KY, Chiang WF, Chang YT, Chen SF, Wang WS, Chuang YN, Lin SY, Chien CY, Chang KP, Chang YS, Yu JS. Variability Assessment of 90 Salivary Proteins in Intraday and Interday Samples from Healthy Donors by Multiple Reaction Monitoring - Mass Spectrometry. Proteomics Clin Appl. 2018 Mar;12(2). |
| Tsai CY, Chi HC, Chi LM, Yang HY, Tsai MM, Lee KF, Huang HW, Chou LF, Cheng AJ, Yang CW, Wang CS, Lin KH. Argininosuccinate synthetase 1 contributes to gastric cancer invasion and progression by modulating autophagy. FASEB J. 2018 May;32(5):2601-2614 |
| Chuang CF, King CE, Ho BW, Chien KY, Chang YC. Unbiased Proteomic Study of the Axons of Cultured Rat Cortical Neurons. J Proteome Res. 2018 May 4;17(5):1953-1966 |
| 2017 |
Hsiao YC, Chi LM, Chien KY, Chiang WF, Chen SF, Chuang YN, Lin SY, Wu CC, Chang YT, Chu LJ, Chen YT, Chia SL, Chien CY, Chang KP, Chang YS, Yu JS. Development of a Multiplexed Assay for Oral Cancer Candidate Biomarkers Using Peptide Immunoaffinity Enrichment and Targeted Mass Spectrometry. Mol Cell Proteomics. 2017 Oct;16(10):1829-1849. |
| Tsai HJ, Chien KY, Liao HR, Shih MS, Lin YC, Chang YW, Cheng JC, Tseng CP. Functional links between Disabled-2 Ser723 phosphorylation and thrombin signaling in human platelets. J Thromb Haemost. 2017 Oct;15(10):2029-2044 |
| Chiu CC, Yeh TH, Lu CS, Huang YC, Cheng YC, Huang YZ, Weng YH, Liu YC, Lai SC, Chen YL, Chen YJ, Chen CL, Chen HY, Lin YW, Wang HL , PARK14 PLA2G6 mutants are defective in preventing rotenone-induced mitochondrial dysfunction, ROS generation and activation of mitochondrial apoptotic pathway Oncotarget. 2017 Sep 15;8(45):79046-79060 |
| Chen TW, Gan RC, Fang YK, Chien KY, Liao WC, Chen CC, Wu TH, Chang IY, Yang C, Huang PJ, Yeh YM, Chiu CH, Huang TW, Tang P. FunctionAnnotator, a versatile and efficient web tool for non-model organism annotation. Sci Rep. 2017 Sep 5;7(1):10430. |
| Hsieh YJ, Chien KY, Yang IF, Lee IN, Wu CC, Huang TY, Yu JS. Oxidation of protein-bound methionine in Photofrin-photodynamic therapy-treated human tumor cells explored by methionine-containing peptide enrichment and quantitative proteomics approach. Sci Rep. 2017 May 2;7(1):1370. |
| Kung YA, Hung CT, Chien KY, Shih SR. Control of the negative IRES trans-acting factor KHSRP by ubiquitination. Nucleic Acids Res. 2017 Jan 9;45(1):271-287. |
| 2016 |
Wang TH, Yu CC, Lin YS, Chen TC, Yeh CT, Liang KH, Shieh TM, Chen CY, Hsueh C. Long noncoding RNA CPS1-IT1 suppresses the metastasis of hepatocellular carcinoma by regulating HIF-1α activity and inhibiting epithelial-mesenchymal transition. Oncotarget. 2016 Jul 12;7(28) |
| Cheng ML, Chi LM, Wu PR, Ho HY. Dehydroepiandrosterone-induced changes in mitochondrial proteins contribute to phenotypic alterations in hepatoma cells. Biochem Pharmacol. 2016 Oct 1;117:20-34. |
| Chen YC, Chi LM, Chow KC, Chiou SH, Fan YH, Ho SP Hsu YC, Hwang YC, Wu MX, Lee WM, Lin SL, Tsang CL, Mao FC. Association of anticardiolipin, antiphosphatidylserine, anti-β2 glycoprotein I, and antiphosphatidylcholine autoantibodies with canine immune thrombocytopenia. BMC Vet Res. 2016 Jun 13;12(1):106. |
| Hsieh SY, Lee CH, Lin CC. Platelet: A friend or foe of patients with hepatoma? Liver Int. 2016 Apr;36(4):615. |
| Chiu CY, Lin G, Cheng ML, Chiang MH, Tsai MH,Lai SH,Wong KS, Hsieh SY.Metabolomic Profiling of Infectious Parapneumonic Effusions Reveals Biomarkers for Guiding Management of Children with Streptococcus pneumoniae Pneumonia. Sci Rep. 2016 Apr 22;6:24930. |
| Cheng HT, Hsieh SY, Sung CM, Pai BC, Liu NJ, Chen CP. Optimizing Human Bile Preparation for Two-Dimensional Gel Electrophoresis. Biomed Res Int. 2016 Feb 4. |
| 2015 |
Chern-Horng Lee, Yu-Jr Lin, Chen-Chun Lin, Cho-Li Yen, Chien-Heng Shen,Chee-Jen Chang and Sen-Yung Hsieh. Pretreatment platelet count early predicts extrahepatic metastasis of human hepatoma. Liver international,. 2015 Oct;35(10):2327-36. |
| Chiu CY, Hsieh SY, Wong KS, Lai SH, Chen JK, Huang JL. The value of total protein in guiding management of infectious parapneumonic effusion by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. J Microbiol Immunol Infect. 2015 Oct;48(5):483-9. |
| Chang KP, Lin SJ, Liu SC, Yi JS, Chien KY, Chi LM, Kao HK, Liang Y, Lin YT, Chang YS, Yu JS. Low-molecular-mass secretome profiling identifies HMGA2 and MIF as prognostic biomarkers for oral cavity squamous cell carcinoma. Sci Rep. 2015 Jul 3;5:11689. |
| Lin CS, Su CC, Hsieh SC, Lu CC, Wu TL, Jia JH, Wu TS, Han CC, Tsai WC, Lu JJ, Lai HC. Rapid identification of Mycobacterium avium clinical isolates by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. J Microbiol Immunol Infect. 2015 Apr;48(2) |
| Chung PJ, Chi LM, Chen CL, Liang CL, Lin CT, Chang YX, Chen CH, Chang YS. MicroRNA-205 targets tight junction-related proteins during urothelial cellular differentiation. Mol Cell Proteomics. 2014 Sep;13(9):2321-36. |
| Fang KH, Kao HK, Chi LM, Liang Y, Liu SC, Hseuh C, Liao CT, Yen TC, Yu JS, Chang KP. Overexpression of BST2 is associated with nodal metastasis and poorer prognosis in oral cavity cancer. Laryngoscope. 2014 Sep;124(9):E354-60. |
| 2014 |
Chih-Yung Chiu, Sen-Yung Hsieh, Kin-Sun Wong, Shen-Hao Lai, Jen-Kun Chen, Jing-Long Huang. The value of total protein in guiding management of infectious parapneumonic effusion by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Journal of Microbiology, Immunology and Infection. 2014 Feb 21. |
| Rabindranath Bera, Chih-Yung Chiou, Ming-Chin Yu, Jei-Ming Peng, Chung-Ru He, Chih-Yun Hsu, Hsiao-Ling Huang, Uda Y. Ho, Shi-Ming Lin, Yu-Jr Lin, and Sen-Yung Hsieh. Functional genomics identified a novel protein tyrosine phosphatase receptor type F-mediated growth inhibition in hepatocarcinogenesis.. Hepatology. 2014 Jun;59(6):2238-50. |
| 2013 |
Ying-Che Chang , Hong-Wen Tang , Suh-Yuen Liang , Tsung-Hsien Pu , Tzu-Ching Meng , Kay-Hooi Khoo , and Guang-Chao Chen . Evaluation of Drosophila Metabolic Labeling Strategies for in Vivo Quantitative Proteomic Analyses with Applications to Early Pupa Formation and Amino Acid Starvation. Journal of Proteome. 2013 12(5): pp 2138–2150. |
| Chang KP, Wang CL, Kao HK, Liang Y, Liu SC, Huang LL, Hseuh C, Hsieh YJ, Chien KY, Chang YS, Yu JS, Chi LM*. Overexpression of caldesmon is associated with lymph node metastasis and poorer prognosis in patients with oral cavity squamous cell carcinoma. Cancer. 2013 Nov 15;119(22):4003-11. |
| Lo SJ, Fan LC, Tasi YF, Lin KY Huang HL, Wang TH, Liu H, Chen TC, Huang SF, Chang CJ, Lin YJ, Yung BY, Hsieh SY. A novel interaction of nucleophosmin with BCL2-associated X protein regulating death evasion and drug sensitivity in human hepatoma cells. Hepatology. 2013 May;57(5):1893-905 |
| Lee Chern-Horng, Hsieh Sen-Yung, Lin Ja-Liang, Liu Maw-Sen and Yen Tzung-Hai. Hepatocellular carcinoma in patients with chronic kidney disease. World J Gastroenterol. 2013 April 28;19(16): 2466-2472. |
| Yuan Kuo-Ching, Fang Jen-Feng, Hsieh Sen-Yung, Shih Hsin-Nung. Comparative Proteomic Analysis of Rodent Plasma and Mesenteric Lymph. Chinese Journal of Physiology. Erratum in: Chin J Physiol. 2013 Jun 30;56(3):163-73. |
| Lee CH, Hsieh SY, Chang CJ, Lin YJ. Comparison of clincal characteristics of combined hepatocellular-cholangiocarcinoma and other primary liver cancers. J Gastroenterol Hepatol. 2013 Jan;28(1):122-7 |
| Chen JT, Ho CW, Chi LM, Chien KY, Hsieh YJ, Lin SJ, Yu JS. Identification of the lamin A/C phosphoepitope recognized by the antibody P-STM in mitotic HeLa S3 cells. BMC Biochem. 2013 Jul 19;14:18. |
| Lin SJ, Chang KP, Hsu CW, Chi LM, Chien KY, Liang Y, Tsai MH, Lin YT, Yu JS. Low-molecular-mass secretome profiling identifies C-C motif chemokine 5 as a potential plasma biomarker and therapeutic target for nasopharyngeal carcinoma. J Proteomics. 2013 Dec 6;94:186-201. |
| 2012 |
Shih Tsung-Chieh, Tien Yin-Jing, Wen Chih-Jen, Yeh Ta-Sen, Yu Ming-Chin, Huang Chia-Hao, Lee Yun-Shien , Yen Tzu-Chen, Hsieh Sen-Yung. MicroRNA-214 downregulation contributes to tumor angiogenesis by inducing secretion of the hepatoma-derived growth factor in human hepatoma. Journal of Hepatology. 2012 Sep;57(3): 584–591 |
| Chen, CPC (Chen, Carl P. C.); Chen RL; Preston JE. The influence of ageing in the cerebrospinal fluid concentrations of proteins that are derived from the choroid plexus, brain, and plasma. Exp Gerontol. 2012 Apr;47(4):323-8 |
| Hsieh Y-J, Chien K-Y, Lin S-Y, Sabu S, Hsu R-M, Chi L-M, Lyu P-C, and Yu J-S*. Photofrin binds to procaspase-3 and mediates photodynamic treatment–triggered methionine oxidation and inactivation of procaspase-3. Cell Death & Disease 2012 July 12, 3: e347 |
| Chen CY, Chi LM, Chi HC, Tsai MM, Tsai CY, Tseng YH, Lin YH, Chen WJ, Huang YH, Lin KH. Stable isotope labeling with amino acids in cell culture (SILAC)-based quantitative proteomics study of a thyroid hormone-regulated secretome in human hepatoma cells. Molecular & Cellular Proteomics. 2012 Apr;11(4):M111.0112 |
| 2011 |
Hsieh Sen-Yung; He Jung-Ru; Yu Ming-Chin; et al. Secreted ERBB3 Isoforms Are Serum Markers for Early Hepatoma in Patients with Chronic Hepatitis and Cirrhosis. J. Proteome Research, 2011, 10(10)4715-4724 |
| Chen R, Vendrell I, Chen CP (陳伯旭), Cash D, O'Toole KG, Williams SA, Jones C, Preston JE, Wheeler JX. Proteomic analysis of rat plasma following transient focal cerebral ischemia. Biomark Med. 2011 Dec;5(6):837-46. |
| Chen CP, Hsu CC, Yeh WL, Lin HC, Hsieh SY, Lin SC, Chen TT, Chen MJ, Tang SF. Optimizing Human Synovial Fluid Preparation for Two-Dimensional Gel Electrophoresis. PROTEOME SCIENCE, 2011, :9 ( 65 )-p.5956-9-65 |
| Hsieh Sen-Yung; He Jung-Ru; Hsu Chih-Yun; et al. Neuregulin/Erythroblastic Leukemia Viral Oncogene Homo log 3 Autocrine Loop Contributes to Invasion and Early Recurrence of Human Hepatoma. HEPATOLOGY 2011,:53(2) p.504-516. |
| Chang KP, Yu JS, Chien KY, Lee CW, Liang Y, Liao CT, Yen TC, Lee LY, Huang LL, Liu SC, Chang YS, Chi LM*. Identification of PRDX4 and P4HA2 as Metastasis-Associated Proteins in Oral Cavity Squamous Cell Carcinoma by Comparative Tissue Proteomics of Microdissected Specimens Using iTRAQ Technology. Journal of Proteome Research, 2011 Nov 4;10(11):4935-47. |
| Huang YC,Wu YR,Tseng MY,Chen YC,Hsieh SY,Chen CM. Increased Prothrombin, Apolipoprotein A-IV, and Haptoglobin in the Cerebrospinal Fluid of Patients with Huntington's Disease. PLOS ONE 2011 Jan 31;6(1):e15809. |
| Wang CN, Chen JY, Sabu S, Chang YL, Chang SD, Kao CC, Peng HH, Chueh HY, Chao AS, Cheng PJ, Lee YS*, Chi LM*, Wang TH*. Elevated amniotic fluid F₂-isoprostane: a potential predictive marker for preeclampsia. Free Radical Biology and Medicine 2011 May 1;50(9):1124-30. |
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