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NEWSLETTER NEWSLETTER
SEPTEMBER 02-4. 組織工程研究中心 SEPTEMBER
2018 2018
Enhanced critical-size calvarial bone healing by ASCs
engineered with Cre/loxP-based hybrid baculovirus
撰稿者：張毓翰教授 校稿者：鄭明輝教授
說明： 研究發現與應用：
Calvarial bone repair remains The transduction of rat ASCs with the hybrid
challenging for adults. Although Cre/loxP-basedbaculovirus enabled robust and
adipose-derived stem cells (ASCs) sustained expression of functional BMP-2 and
hold promise to heal bone defects, SDF-1 was demonstrated.
use of ASCs for critical-size
calvarial bone repair is ineffective. Expression of BMP-2 or SDF-1 alone failed
Stromal cell-derived factor 1 to effectively induce rat ASCs osteogenesis and
(SDF-1) is a chemokine capable healing of critical-size calvarial bone defects.
of triggering stem cell migration.
Although recombinant SDF- Prolonged BMP-2/SDF-1 co-expression in
1 protein is co-delivered with ASCs synergistically activated both Smad and
other molecules including BMP- ERK1/2 pathways and hence potentiated the
2 to facilitate calvarial bone osteogenesis.
repair, these approaches did not
yield satisfactory healing. This MP-2/SDF-1-expressing ASCs/scaffold
study aimed to exploit a newly constructs potently healed the critical-size(6 Figure: μCT analysis of in vivo calvarial bone repair. The rat ASCs were transduced
developed Cre/loxP-based hybrid mm) calvarial bone defects (filling ≈70% and seeded to porous gelatin scaffold (5 × 106 cells/scaffold), cultured overnight and
baculovirus for efficient gene of defect volume), which considerably implanted into critical-size (6 mm in diameter) defect at the calvaria of 6-week-old
delivery and prolonged transgene outperformed the calvarial bone repair using SD rats (n = 4 for Mock and n = 5 for the LEBW, LESW and LEBW/LESW groups).
expression in ASCs.
BMP-2/SDF-1 delivered with biomaterial- The rats were scanned with a μCT imaging system at different weeks post-surgery.
based scaffolds.
(Lo SC, et al. Biomater als. 2017 Apr;124:1-11. doi: 10.1016. SCI; IF =8.402;
This study implicated the potential of
Cre/loxP-based hybrid baculovirus vector for Engineering, Biomedical 2/77)
ASCsengineering and calvarial bone healing.
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