Page 78 - 醫研部6月份電子報

•Figure 2. Effects of RSV on MPO activities, wet/dry weight ratios,

histopathologic features, and neutrophil infiltration and activation
of lungs in mice after LPS-induced ALI. C57BL/6 mice were treated

with RSV (100 mg/kg body weight, intraperitoneally) or an equal

volume of vehicle. Six h after instilling LPS or 0.9% saline into the
trachea, the mouse lungs were harvested for measurement of (A) MPO

activityand(B)wet/dryweightratios.(C)Representative photomicrographs

of lungs from (I) sham-operated mice injected with vehicle, (II) sham-
operated mice injected with RSV, (III) ALI animals injected with vehicle,

and (IV) ALI animals injected with RSV. Mouse lungs were resected and

subjected to hematoxylin and eosin (HE) staining as well as

immunofluorescent detection for lymphocyte antigen 6complex locus
G6D (Ly6G) and 4-hydroxynonenal (4-HNE). The 4′,6-diamidino-2-

phenylindole (DAPI) stain was used for localizing nuclear DNA. RSV

significantly attenuated pulmonary damage, neutrophil infiltration, and
oxidative stress.

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