Page 77 - 醫研部6月份電子報

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•Figure 1. RSV significantly inhibits superoxide anion release, elastase

release, intracellular superoxide formation, CD11b expression, and cell

adhesion in activated human neutrophils. Human neutrophils were
incubated with DMSO (0.1%, as a control) or RSV (3–50 μM) for 5 min prior

to cell activation. (A, B) The release of superoxide anion and elastase were

respectively detected spectrophotometrically by using ferricytochrome c and
elastase substrate. (C) Hydroethidine-labeled neutrophils were stimulated

using fMLF/CB for another 5 min. Intracellular superoxide levels were

determined according to intracellular fluorescence intensity, as measured by

flow cytometry. (D) CD11b expression was induced by fMLF/CB for 5 min.
The fluorescence intensity of FITC-labeledanti-CD11b was monitored using

flow cytometry. (E, F) Hoechst 33342 (1 ng/mL)-stained neutrophils were

incubated with RSV (3–50 μM) for 5 min, followed by activation with
fMLF/CB for 15 min. The LPS-treated endothelial cells were coincubated with

previously labeled neutrophils for 15 min. The adherent neutrophils on

endothelial cells were calculated by a motorized inverted microscope.

Department of Medical Research & Development, Linkou Newsletter 77