Regenerating Cartilages by Engineered ASCs: Prolonged TGF-β3/BMP-6 Expression Improved Articular Cartilage Formation and Restored Zonal Structure

Chia-Hsin Lu, Tsung-Szu Yeh, Chia-Lin Yeh, Yu-Hua Dean Fang, Li-Yu Sung, Shih-Yeh Lin, Tzu-Chen Yen Yu-Han Chang, and Yu-Chen Hu (Mol Ther. 2014; 22(1):186-95, IF=6.425)

Osteogenic differentiation of adipose-derived stem cells and calvarial defect repair using baculovirus-mediated co-expression of BMP-2 and miR-148b

鄭明輝教授

顏宗海教授

(TH Yen, et al, J.Cell. Mol. Med, 2015;19:463-73, IF=4.014)

馬惠康教授

Cultivated autologous oral mucosal ocular epithelial transplantation for the treatment of surface diseases: Phase Ib clinical trial
(TFDA approved, trial no.: 1040027480)

Preservation of epithelial progenitor cells from collagenase digested
oral mucosa during ex vivo cultivation
Yi-Jen Hsueh, Shiang-Fu Huang, Jui-Yang Lai, Hung-Chi Chen*, Shih-Chieh Ma, Kevin Sheng-Kai Ma, Sung-En Wu, Tze-Kai Wang, Jan-Kan Chen, Chyong-Huey Lai David Hui-Kang Ma*
(Sci Rep. 2016 Nov 8;6:36266, IF=4.259)

Comparison of original and modified protocols for the ex vivo cultivation of human oral mucosal epithelial cells (OMECs).
Left panel: In the original protocol, oral epithelium was isolated from oral mucosa with dispase II. Suspended OMECs were then dissociated with trypsin-EDTA, re-suspended and cultivated on de-epithelialized amniotic membrane (AM) with 3T3 fibroblasts as a feeder layer, in SHEM containing fetal bovine serum (FBS). Right panel: In the modified protocol, oral mucosal tissues were treated with collagenase A. The resulting cell aggregates were grown on AM without a 3T3 feeder layer. In addition, FBS was replaced with PLTMax. Two days after seeding, the culture medium was changed to serum-free medium (EpiLife) to prevent the overgrowth of submucosal fibroblasts.

Epithelial progenitors are preserved in collagenase-digested oral mucosa
Yi-Jen Hsueh, Shiang-Fu Huang, Jui-Yang Lai, Hung-Chi Chen, Shih-Chieh Ma, Kevin Sheng-Kai Ma, Sung-En Wu, Tze-Kai Wang, Jan-Kan Chen, Chyong-Huey Lai David Hui-Kang Ma
(manuscript under submission)

Preservation of Human Limbal Epithelial Progenitor Cells on Carbodiimide Cross-linked Amniotic Membrane via Integrin-Linked Kinase-mediated Wnt Activation
David Hui-Kang Ma, Hung-Chi Chen,Kevin Sheng-Kai Ma, Jui-Yang Lai, Unique Yang, Lung-Kung Yeh, Yi-Jen Hsueh, Wing-Keung Chu, Chyong-Huey Lai, and Jan-Kan Chen
(Acta Biomaterialia. 2015 accepted, IF=6.025)

We demonstrated the superior capability of carbodiimide cross-linked denudedamniotic membrane (CLDAM) than natural DAM to preserve limbo-corneal epithelialprogenitor cells in vitro, then we used CLDAM as a simulated epithelial basementmembrane (EBM) to study how EBM maintains limbal epithelial stem cells (LESCs).
We found that integrin-linked kinase (ILK) is an important mediator that transferssurvival signals detected by integrin β1 to the Wnt/β-catenin pathway, which in turnup-regulates deltaNp63α, a master gene that regulates LESC function. The roughersurface of the limbal EBM suggests that the surface complexity of the LESC nichemay be important in regulating LESC function, which is triggered by the recognitionof topographic cues by integrin β1, followed by activation of the ILK/Wnt/β-catenin/p63 cascade.

生物材料與影像分析組

賴伯亮教授

曹中侃主任

Trachea Tissue Engineering

黃嫆茹副教授

A comparative study of the chondrogenic potential between synthetic and natural scaffolds in an in vivo bioreactor
Jung-Ju Huang, Shu-Rui Yang, I-Ming Chu, Eric M Brey, Hui-Yi Hsiao and Ming-Huei Cheng
(Sci. Technol. Adv. Mater. 2013;14:054403 IF=3.513)

吳杰才主任