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論文 |
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Chen Yiyang(陳苡揚醫師), Lina Marie Hoffmeister, Yasmin Zaun, Lucas Arnold, Kurt Werner Schmid, Bernd Giebel, Ludger Klein-Hitpass, Helmut Hanenberg, Anthony Squire, H. Christian Reinhardt, Ulrich Dührsen, Stefanie Bertram and Maher Hanoun* (2020). "Acute myeloid leukemia–induced remodeling of the human bone marrow niche predicts clinical outcome." Blood Advances 4(20): 5257-5268.
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從急性骨髓性白血病(AML)患者的外周血及骨髓分離單個核細胞(PBMCs and BMCs)後，利用磁珠套組去除表現CD45的細胞，再利用流式細胞分選儀篩選表達CD45－CD235a－CD31－CD146low/＋CD271＋的間質幹細胞和前驅細胞(MSPCs)，以及表達CD45－CD235a－CD31－CD146－CD271－的基質細胞(stromal cells)進行實驗。
在MSPCs骨細胞分化過程中加入不同AML細胞系(KG-1、U937、THP-1、OCI-AML3、MOLM-13)共同培養後，以流式細胞分析儀檢測MSPCs中的細胞質β-catenin水平來評估WNT /β-catenin途徑的活性。
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| 2 |
Lee I-Neng(李一能博士), Yang Jen-Tsung, Hsieh Ming-Ju, Huang Cheng, Huang Hsiu-Chen, Ku Yu-Ju, Wu Yu-Ping, Huang Kuan-Chieh and Chen Jui-Chieh* (2020). "Knockdown of Amphiregulin Triggers Doxorubicin-Induced Autophagic and Apoptotic Death by Regulating Endoplasmic Reticulum Stress in Glioblastoma Cells." Journal of Molecular Neuroscience 70(10): 1461-1470.
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| 利用共軛焦顯微鏡拍攝膠質瘤細胞(U87MG) cleavage caspase 3之免疫螢光染色結果，以觀察抑制Amphiregulin (AREG)的表現是否有助於提升膠質母細胞瘤對Doxorubicin藥物的感受性，進一步評估AREG作為腦癌多形性母細胞瘤治療目標之可行性。
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Yang Chao-Hui, Hwang Chung-Feng*, Chuang Jiin-Haur, Lian Wei-Shiung, Wang Feng-Sheng, Huang Ethan I. and Yang Ming-Yu* (2020). "Constant Light Dysregulates Cochlear Circadian Clock and Exacerbates Noise-Induced Hearing Loss." International Journal of Molecular Sciences 21(20): 7535.
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| 以共軛焦顯微鏡拍攝小鼠耳蝸組織免疫螢光染色結果，利用抗CTBP2抗體標記內毛細胞突觸帶，以及抗4-HNE抗體偵測外毛細胞脂質過氧化物，觀察生理日夜節奏(circadian rhythms)在噪音性聽力損失中的影響。
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Chen Miao-Fen*(陳妙芬醫師), Lu Ming-Shian(呂明憲醫師), Hsieh Ching-Chuan and Chen Wen-Cheng (2021). "Porphyromonas gingivalis promotes tumor progression in esophageal squamous cell carcinoma." Cellular Oncology 44(2): 373-384.
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以流式細胞分析儀檢測牙齦紫質單孢菌感染的食道鱗狀細胞癌細胞(CE81T及TE2)中CD11b、Gr1、CD44、PD-L1、ALDH1的表達。
以共軛焦顯微鏡拍攝免疫螢光染色結果，檢測食道鱗狀細胞癌細胞(CE81T及TE2)受到牙齦紫質單孢菌感染後，其癌轉移(EMT)與癌幹細胞(CSC)指標蛋白的變化與後續訊號傳遞機制，並進一步評估骨化三醇(Calcitriol)在體外治療牙齦紫質單孢菌誘變食道癌的效果與臨床應用之策略。
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Teng Chih-Chuan(鄧志娟老師), Tung Shui-Yi, Lee Ko-Chao, Lee Kam-Fai, Huang Wen-Shih, Shen Chien-Heng, Hsieh Meng-Chiao, Huang Cheng-Yi, Sheen Jiunn-Ming and Kuo Hsing-Chun*(郭星君老師) (2021). "Novel regulator role of CIL-102 in the epigenetic modification of TNFR1/TRAIL to induce cell apoptosis in human gastric cancer." Food and Chemical Toxicology 147: 111856.
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| 以流式細胞分析儀檢測抗菌藥物CIL-102對胃癌細胞(AGS)的影響，包含分析細胞凋亡Annexin V表現、活性氧(ROS)濃度、Ca2+釋放速率、細胞週期比例。
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Tung Shui-Yi(董水義醫師), Lee Ko-Chao, Lee Kam-Fai, Yang Ya-Ling, Huang Wen-Shih, Lee Li-Ya, Chen Wan-Ping, Chen Chin-Chu, Teng Chih-Chuan, Shen Chien-Heng, Hsieh Meng-Chiao, Huang Cheng-Yi, Sheen Jiunn-Ming and Kuo Hsing-Chun*(郭星君老師) (2021). "Apoptotic mechanisms of gastric cancer cells induced by isolated erinacine S through epigenetic histone H3 methylation of FasL and TRAIL." Food & Function.
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| 以流式細胞分析儀檢測猴頭菇菌絲體活性產物猴頭素 S (Erinacine S)對胃癌細胞(AGS)的影響，包含分析細胞凋亡Annexin V表現、活性氧(ROS)濃度、細胞週期比例。
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Lin Chin-Kuo(林進國醫師), Huang Tzu-Hsiung, Yang Cheng-Ta and Shi Chung-Sheng*(石宗憲老師) (2021). "Roles of lung-recruited monocytes and pulmonary Vascular Endothelial Growth Factor (VEGF) in resolving Ventilator-Induced Lung Injury (VILI)." PLOS ONE 16(3): e0248959.
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| 以流式細胞分析儀檢測由模擬肺呼吸機誘發肺損傷(VILI)的小鼠模型所收集的肺部細胞中CD11b、F4/80、Ly6C的表達水平，鑑定單核細胞(monocytes)表型。
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