採檢時應避免來自皮膚表面常在菌叢的污染,如Corynebacterium spp.,α,γ-Streptococcus spp., Coagulase-negative staphylococci, Bacillus spp., Propionbacterium spp.等。若採檢及傳送過程適當,則大多檢體可找到致病原。常見的致病菌有： (1)癤：S. aureus (2)Impetigo： S. aureus, Group A Streptococcus (3)Ulcer：S. aureus, Group A Streptococcus, G(-)Bacilli, Enterobacteriaceae,Pseudomonas sp.及其他Pseudomonas spp. (4)Burn： S. aureus, Enterococci, P. aeruginosa及其他Pseudomonas spp. (5)Sinus：Actinomyces, Bacteroides spp., Enterococci (6)Fistula：小腸內的normal flora (7)Bite：Bite：Pasteurella multocida、Capnocytophaga spp.、Eikenella corrodens(動物咬傷),, Streptobacillus moniliformis (鼠咬傷), Fusobacterium (人咬傷) (8)Deep seated abscess： Bacteroides, Streptococcus, Enterococcus, Enterobacteriaceae