Dear Friends
本週四(10/19)Journal Club
由蔡熒煌醫師研究群的洪雅琪小姐
為大家報告一篇選自Proteomics的文章,題目為:
Different techniques for urinary protein analysis of normal and lung cancer
patients
Abstract
Many components in urine are useful in clinical diagnosis and urinary proteins
are known as important components to define many diseases such as proteinuria,
kidney, bladder and urinary tract diseases. In this study, we focused on the
comparison of different sample preparation methods for isolating urinary
proteins prior to protein analysis of pooled healthy and lung cancer patient
samples. Selective method was used for preliminary investigation of some
putative urinary protein markers. Urine samples were passed first through a gel
filtration column (PD-10 desalting column) to remove high salts and subsequently
concentrated. Remaining interferences were removed by ultrafiltration or four
precipitation methods. The analysis of urinary proteins by high-performance
liquid chromatography and sodium dodecyl sulfate-polyacrylamide gel
electrophoresis showed many similarities in profiles among preparation methods
and a few profiles were different between normal and lung cancer patients. In
contrast, the results of two-dimensional gel electrophoresis (2-DE) showed more
distinctly different protein patterns. Our finding showed that the sequential
preparation of urinary proteins by gel filtration and ultrafiltration could
retain most urinary proteins which demonstrated the highest protein spots on 2-D
gels and able to identify preliminary urinary protein markers related to cancer.
Although sequential preparation of urine samples by gel filtration and protein
precipitation resulted in low amounts of proteins on 2-D gels, high Mr proteins
were easily detected. Therefore, there are alternative choices for urine sample
preparation for studying the urinary proteome and identifing urinary protein
markers important for further preclinical diagnostic and therapeutic
applications.
地點:心內科會議室,J1電梯旁
時間:10/19週四下午4:30
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